Enterovirus 71 (EV71) IgM ELISA Kit

Takaitaccen Bayani:

Enterovirus 71 IgM (EV71-IgM) ELISA kit shine gwajin immunosorbent mai alaƙa da enzyme don gano ingantattun ƙwayoyin rigakafi na IgM-class zuwa Enterovirus 71 a cikin ƙwayar ɗan adam ko plasma.An yi niyya don amfani da shi a cikin dakunan gwaje-gwaje na asibiti don ganowa da sarrafa marasa lafiya da ke da alaƙa da kamuwa da cuta tare da Enterovirus 71.

Mutum enterovirus 71 (EV71), sabon memba na Enteroviridae, shine sanadi na yau da kullum na cutar hannu, ƙafa da kuma baki (HFMD) kuma wani lokaci yana hade da cututtuka masu tsanani na tsakiya.Ciwon cututtuka yakan yi ƙamari a watan Yuni da Yuli.Kamuwa da EV71 na iya zama duka asymptomatic ko yana iya haifar da gudawa da rashes.Yara 'yan kasa da biyar suna cikin haɗari mafi girma don kamuwa da cuta mai tsanani fiye da manya da yara manya.

Ana yada EV71 daga mutum zuwa mutum ta hanyar tuntuɓar juna kai tsaye da kuma ta hanyar gurɓata hannaye ko abubuwa masu ɗauke da najasa.Sigar hanci da makogwaro, miya ko ruwa daga blisters kuma na iya yada cutar.

Ana iya keɓe EV71 kuma a gano shi a cikin maƙogwaro da samfuran stool ta amfani da gwajin PCR.An gano kwayar cutar RNA a cikin ruwan vesicle na fata, jini da fitsari.Tun da farko kuma za a iya samun sauƙin ganewar asali ta amfani da takamaiman gwaje-gwajen jini na EV71, gami da gwaje-gwaje na musamman don rigakafin IgM.


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Ka'ida

Wannan kit ɗin yana gano Enterovirus 71 IgM antibody (EV71-IgM) a cikin jinin mutum ko samfuran plasma, polystyrene microwell tubes an riga an rufe su da ƙwayoyin rigakafi da aka kai ga furotin na immunoglobulin M na ɗan adam (anti-μ sarkar) . da aka bincika, ana iya kama ƙwayoyin rigakafi na IgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a haɗa su ba (ciki har da takamaiman ƙwayoyin rigakafi na IgG) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da ƙwayoyin rigakafi na EV71 IgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da halayen haɓaka launi. don samar da abu mai shuɗi, ƙara 50μL na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na EV71-IgM antibody a cikin samfurin an ƙaddara ta mai karanta microplate.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida Enzyme hade da immunosorbent assay
Nau'in Hanyar ɗauka
Takaddun shaida CE
Misali jinin mutum / plasma
Ƙayyadaddun bayanai 48T/96T
Yanayin ajiya 2-8 ℃
Rayuwar rayuwa watanni 12

Bayanin oda

Sunan samfur Kunshi Misali
Enterovirus 71 (EV71) IgM ELISA Kit 48T/96T jinin mutum / plasma

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