Enterovirus 71 (EV71) IgM ELISA Kit
Ka'ida
Wannan kit ɗin yana gano Enterovirus 71 IgM antibody (EV71-IgM) a cikin jinin mutum ko samfuran plasma, polystyrene microwell tubes an riga an rufe su da ƙwayoyin rigakafi da aka kai ga furotin na immunoglobulin M na ɗan adam (anti-μ sarkar) . da aka bincika, ana iya kama ƙwayoyin rigakafi na IgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a haɗa su ba (ciki har da takamaiman ƙwayoyin rigakafi na IgG) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da ƙwayoyin rigakafi na EV71 IgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da halayen haɓaka launi. don samar da abu mai shuɗi, ƙara 50μL na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na EV71-IgM antibody a cikin samfurin an ƙaddara ta mai karanta microplate.
Siffofin Samfur
Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali
Ƙayyadaddun samfur
Ka'ida | Enzyme hade da immunosorbent assay |
Nau'in | Hanyar ɗauka |
Takaddun shaida | CE |
Misali | jinin mutum / plasma |
Ƙayyadaddun bayanai | 48T/96T |
Yanayin ajiya | 2-8 ℃ |
Rayuwar rayuwa | watanni 12 |
Bayanin oda
Sunan samfur | Kunshi | Misali |
Enterovirus 71 (EV71) IgM ELISA Kit | 48T/96T | jinin mutum / plasma |