Enterovirus 71(EV71) IgG ELISA Kit

Takaitaccen Bayani:

Human Enterovirus 71 IgG ELISA Kit shine gwajin immunosorbent mai alaƙa da enzyme don gano ingantattun ƙwayoyin rigakafi na IgG-class zuwa Enterovirus 71 a cikin ƙwayar ɗan adam ko plasma.An yi niyya don amfani da shi a cikin dakunan gwaje-gwaje na asibiti don ganowa da sarrafa marasa lafiya da ke da alaƙa da kamuwa da cuta tare da Enterovirus 71.

Babban bayyanar asibiti na kamuwa da cutar EV71 shine cutar hannu, ƙafa da kuma baki.Wasu yara masu kamuwa da cutar EV71 suna zuwa tare da pharyngitis na herpetic, kuma a lokuta masu tsanani, ƙwayar cuta ta ƙwayar cuta, ƙwayar cuta ta kwayar cuta, edema na huhu da jini na huhu.

Ƙayyadaddun ganewar asali na EV71 kamuwa da cuta ya dogara ne akan gano EV71-RNA, EV71-IgM da EV71-IgG antibodies a cikin jini, ko EV71-RNA a cikin samfurori na swab.

EV71-IgM muhimmiyar alama ce ta kamuwa da cuta ta farko ko ta kwanan nan kuma tana sauƙaƙe ganowa da wuri da maganin kamuwa da cutar EV71.EV71-IgG alama ce mai mahimmanci don ganewar asali na kamuwa da cuta kuma ana iya amfani dashi don bincike na annoba da kimanta rigakafin.Ana iya amfani da shi don bincike na annoba da kuma kimanta tasirin rigakafin.Canjin antibody titers a duka warkewa da m jini kuma iya ƙayyade kamuwa da cuta EV71, kuma idan titer na serum antibody ya karu geometrically da 4 a dawo da lokacin idan aka kwatanta da m lokaci, to, shi za a iya yi hukunci a matsayin EV71 gabatar kamuwa da cuta.


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Ka'ida

Wannan kit ɗin yana gano Enterovirus 71 IgG antibody (EV71-IgG) a cikin jini na ɗan adam ko samfuran plasma, an riga an riga an rufe su da polystyrene microwell tube da Enterovirus 71 antigen.Bayan an fara ƙara samfuran jini ko na plasma da za a bincika, daidaitattun takamaiman ƙwayoyin rigakafi (EV71-IgG-Ab & wasu IgM-Ab) waɗanda ke cikin samfuran marasa lafiya suna ɗaure ga antigens a cikin lokaci mai ƙarfi, kuma za a cire sauran abubuwan da ba a ɗaure su ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) -haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe anti-dan adam IgG zai amsa musamman kawai tare da EV71 IgG antibodies.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (EV71 Ag) - (EV71-IgG) - (anti-mutum IgG-HRP) immunocomplex, bayan wanke farantin, da TMB substrate da aka kara don ci gaban launi, da kuma HRP da alaka da hadaddun catalyzes da launi developer dauki. don samar da abu mai shuɗi, ƙara 50μL na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na EV71-IgG antibody a cikin samfurin an ƙaddara ta mai karanta microplate.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida Enzyme hade da immunosorbent assay
Nau'in Hanyar kai tsaye
Takaddun shaida CE
Misali jinin mutum / plasma
Ƙayyadaddun bayanai 48T/96T
Yanayin ajiya 2-8 ℃
Rayuwar rayuwa watanni 12

Bayanin oda

Sunan samfur Kunshi Misali
Enterovirus 71(EV71) IgG ELISA Kit 48T/96T jinin mutum / plasma

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