Enterovirus 71(EV71) IgG ELISA Kit
Ka'ida
Wannan kit ɗin yana gano Enterovirus 71 IgG antibody (EV71-IgG) a cikin jini na ɗan adam ko samfuran plasma, an riga an riga an rufe su da polystyrene microwell tube da Enterovirus 71 antigen.Bayan an fara ƙara samfuran jini ko na plasma da za a bincika, daidaitattun takamaiman ƙwayoyin rigakafi (EV71-IgG-Ab & wasu IgM-Ab) waɗanda ke cikin samfuran marasa lafiya suna ɗaure ga antigens a cikin lokaci mai ƙarfi, kuma za a cire sauran abubuwan da ba a ɗaure su ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) -haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe anti-dan adam IgG zai amsa musamman kawai tare da EV71 IgG antibodies.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (EV71 Ag) - (EV71-IgG) - (anti-mutum IgG-HRP) immunocomplex, bayan wanke farantin, da TMB substrate da aka kara don ci gaban launi, da kuma HRP da alaka da hadaddun catalyzes da launi developer dauki. don samar da abu mai shuɗi, ƙara 50μL na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na EV71-IgG antibody a cikin samfurin an ƙaddara ta mai karanta microplate.
Siffofin Samfur
Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali
Ƙayyadaddun samfur
Ka'ida | Enzyme hade da immunosorbent assay |
Nau'in | Hanyar kai tsaye |
Takaddun shaida | CE |
Misali | jinin mutum / plasma |
Ƙayyadaddun bayanai | 48T/96T |
Yanayin ajiya | 2-8 ℃ |
Rayuwar rayuwa | watanni 12 |
Bayanin oda
Sunan samfur | Kunshi | Misali |
Enterovirus 71(EV71) IgG ELISA Kit | 48T/96T | jinin mutum / plasma |