Toxoplasma IgG ELISA Kit

Takaitaccen Bayani:

Toxoplasma IgG ELISA Kit shine gwajin immunosorbent mai alaƙa da enzyme don gano ingantattun ƙwayoyin rigakafi na IgG-class zuwa Toxoplasma a cikin jini na ɗan adam ko plasma.An yi nufin amfani da shi a cikin dakunan gwaje-gwaje na asibiti don ganewar asali da kula da marasa lafiya da ke da alaƙa da kamuwa da cuta tare da Toxoplasma.Gabaɗaya, haɓakar ƙwayoyin rigakafi na Toxoplasma IgM na farko yana bayyana bayan kamuwa da cutar Toxoplasma, wanda za a iya gano shi kusan kwanaki 6 bayan kamuwa da cuta, sannan a hankali ya tashi ya zauna na makonni da yawa zuwa watanni, sannan a hankali yana raguwa har sai ya ɓace.Kyakkyawan rigakafin IgG yana nuna kamuwa da cutar Toxoplasma gondii da ta gabata, yawanci na yau da kullun, amma kuma yana iya zama m.Bayan gwajin farko na rigakafin IgG, ana maimaita irin wannan gwajin makonni 2-3 bayan haka kuma idan an sami ƙaruwa sau 4 ko fiye a cikin ƙarfin ƙwayoyin cuta, ana buƙatar ƙarin ganewar asali.

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Cikakken Bayani

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Ka'ida

Wannan kit ɗin yana gano Toxoplasma lgG antibody (TOX-lgG) a cikin jini na ɗan adam ko samfuran plasma, an riga an riga an rufe su da polystyrene microwell tube da Toxoplasma antigen.Bayan an fara ƙara samfuran jini ko na plasma da za a bincika, daidaitattun takamaiman ƙwayoyin rigakafi (TOX-lgG-Ab & wasu lgM-Ab) da ke cikin samfuran marasa lafiya suna ɗaure ga antigens a lokaci mai ƙarfi, kuma za a cire sauran abubuwan da ba a ɗaure su ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) -haɗe-haɗe na lgG na ɗan adam zai amsa musamman tare da ƙwayoyin rigakafi na TOX lgG.Bayan wanke don cire unbound HRP-conjugate , chromogen mafita an ƙara a cikin rijiyoyin.A gaban (TOX Ag) - (TOX-lgG) - (anti-human lgG-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da halayen haɓaka launi. don samar da abu mai shuɗi, ƙara 50 µ I na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na TOX-lgG antibody a cikin samfurin an ƙaddara ta mai karanta microplate.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida	Enzyme hade da immunosorbent assay
Nau'in	Hanyar kai tsaye
Takaddun shaida	NMPA
Misali	jinin mutum / plasma
Ƙayyadaddun bayanai	48T/96T
Yanayin ajiya	2-8 ℃
Rayuwar rayuwa	watanni 12