Kwayar cutar kyanda (MV) IgG ELISA Kit

Wannan kit ɗin yana gano ƙwayar cutar kyanda IgG antibody (MV-IgG) a cikin jini na ɗan adam ko samfuran plasma, polystyrene microwell tubes an riga an rufe su da kwayar cutar kyanda.Bayan an fara ƙara samfuran jini ko na plasma da za a bincika, daidaitattun takamaiman ƙwayoyin rigakafi (MV-IgG-Ab & wasu IgM-Ab) waɗanda ke cikin samfuran marasa lafiya suna ɗaure ga antigens a cikin lokaci mai ƙarfi, kuma za a cire sauran abubuwan da ba a ɗaure su ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) -haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe-haɗe anti-dan adam IgG zai mayar da martani kawai tare da MV IgG antibodies.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (MV Ag) - (MV-IgG) - (anti-mutum IgG-HRP) immunocomplex, bayan wanke farantin, da TMB substrate da aka kara don ci gaban launi, da kuma HRP da alaka da hadaddun catalyzes da launi developer dauki. don samar da abu mai launin shuɗi, ƙara 50μl na Stop Solution, da kuma juya launin rawaya. Kasancewar shayarwar MV-IgG antibody a cikin samfurin an ƙaddara ta mai karatu na microplate.