Human Cytomegalovirus IgM ELISA Kit
Ka'ida
Wannan kit ɗin yana gano ɗan adam cytomegalovirus IgM antibody (HCMV-IgM) a cikin jinin mutum ko samfuran plasma, polystyrene microwell tubes an riga an rufe su da ƙwayoyin rigakafi da aka kai ga furotin na immunoglobulin M na ɗan adam (anti-µ sarkar).Bayan an fara ƙara samfuran jini ko plasma da za a bincika, za a iya kama ƙwayoyin rigakafi na IgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a ɗaure ba (ciki har da takamaiman ƙwayoyin IgG) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da ƙwayoyin rigakafi na HCMV IgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-µ) (HCMV-lgM) (HCMV-lgM) (HCMV Ag-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da amsawar masu haɓaka launi haifar da shuɗin abu, ƙara 50 µI na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na HCMV-IgM antibody a cikin samfurin an ƙaddara ta mai karanta microplate.
Siffofin Samfur
Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali
Ƙayyadaddun samfur
Ka'ida | Enzyme hade da immunosorbent assay |
Nau'in | Hanyar ɗauka |
Takaddun shaida | NMPA |
Misali | jinin mutum / plasma |
Ƙayyadaddun bayanai | 48T/96T |
Yanayin ajiya | 2-8 ℃ |
Rayuwar rayuwa | watanni 12 |
Bayanin oda
Sunan samfur | Kunshi | Misali |
Human Cytomegalovirus IgM ELISA Kit | 48T/96T | jinin mutum / plasma |