Herpes Simplex cutar IgM ELISA Kit

Wannan kit ɗin yana gano Herpes Simplex Virus I lgM antibody (HSV1-lgM) a cikin jini na ɗan adam ko samfuran plasma, an riga an riga an rufe su polystyrene microwell tare da ƙwayoyin rigakafi da aka kai ga sunadaran immunoglobulin M na ɗan adam (anti-µ sarkar).Bayan an fara ƙara samfuran jini ko plasma da za a bincika, za a iya kama ƙwayoyin rigakafin lgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a ɗaure ba (ciki har da takamaiman lgG ƙwayoyin rigakafi) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da ƙwayoyin rigakafi na HSV1 lgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da amsawar masu haɓaka launi zuwa ga masu haɓaka launi. haifar da shuɗin abu, ƙara 50 µ I na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na HSV1-lgM antibody a cikin samfurin an ƙaddara ta mai karanta microplate.