Hepatitis E Virus IgM ELISA Kit
Ka'ida
Wannan kit ɗin yana gano cutar hanta E IgM antibody (HEV-IgM) a cikin jini na ɗan adam ko samfuran plasma, an riga an rufe su da polystyrene microwell tube tare da ƙwayoyin rigakafi da aka kai ga furotin na immunoglobulin M na ɗan adam (anti-μ sarkar).Bayan an fara ƙara samfuran jini ko plasma da za a bincika, za a iya kama ƙwayoyin rigakafi na IgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a ɗaure ba (ciki har da takamaiman ƙwayoyin IgG) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da ƙwayoyin rigakafi na HEV IgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, bayan wanke farantin, da TMB substrate da aka kara don ci gaban launi, da kuma HRP da alaka da hadaddun catalyzes da launi developer dauki zuwa. haifar da blue abu, ƙara 50μl na Tsaida Magani, kuma juya rawaya.Kasancewar abin sha na HEV-IgM antibody a cikin samfurin an ƙaddara ta mai karanta microplate.
Siffofin Samfur
Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali
Ƙayyadaddun samfur
Ka'ida | Enzyme hade da immunosorbent assay |
Nau'in | Hanyar ɗauka |
Takaddun shaida | CE |
Misali | jinin mutum / plasma |
Ƙayyadaddun bayanai | 48T/96T |
Yanayin ajiya | 2-8 ℃ |
Rayuwar rayuwa | watanni 12 |
Bayanin oda
Sunan samfur | Kunshi | Misali |
Hepatitis E Virus IgM ELISA Kit | 48T/96T | jinin mutum / plasma |