Kwayar cutar Hepatitis D IgM ELISA Kit

Wannan kit ɗin yana gano ƙwayar cutar hanta D IgM antibody (HDV-IgM) a cikin jini na ɗan adam ko samfuran plasma, an riga an rufe su da polystyrene microwell tube tare da ƙwayoyin rigakafi da aka kai ga furotin na immunoglobulin M na ɗan adam (anti-μ sarkar).Bayan an fara ƙara samfuran jini ko plasma da za a bincika, za a iya kama ƙwayoyin rigakafi na IgM a cikin samfurin, kuma za a cire sauran abubuwan da ba a ɗaure ba (ciki har da takamaiman ƙwayoyin IgG) ta hanyar wankewa.A mataki na biyu, HRP (horseradish peroxidase) - antigens masu haɗaka zasu amsa musamman tare da rigakafin HDV IgM.Bayan wankewa don cire unbound HRP-conjugate, ana ƙara maganin chromogen a cikin rijiyoyin.A gaban (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, bayan wanke farantin, an ƙara TMB substrate don haɓaka launi, kuma HRP da aka haɗa da hadaddun yana haifar da amsawar mai haɓaka launi haifar da blue abu, ƙara 50μl na Tsaida Magani, kuma juya rawaya.Kasancewar shayarwar rigakafin HDV-IgM a cikin samfurin an ƙaddara ta mai karanta microplate.