Anti-SARS-CoV-2 Neutralizing Antibody Test Kit (ELISA)

Kit ɗin yana amfani da ƙa'idar gwajin gasa don gano ƙwayoyin rigakafin rigakafin cutar SARS-CoV-2 a cikin samfuran jini ko samfuran plasma.Na farko, samfurin da za a gwada, ingantaccen iko, da kuma mummunan iko ana haɗe su da HRP-RBD ta yadda antibody neutralizing ya ɗaure zuwa HRP-RBD, sa'an nan kuma an ƙara cakuda a cikin farantin kama wanda aka riga aka rufe da furotin hACE2.HRP-RBD ba za a ɗaure shi don kawar da antibody ba kamar yadda duk wani HRP-RBD da ke daure ga antibody wanda ba ya tsaka-tsaki za a kama shi akan farantin mai rufi, yayin da hadaddun HRP-RBD da ke daure don neutralizing antibody ana cirewa yayin wankewa.An ƙara maganin TMB don haɓaka launi.A ƙarshe, an ƙara maganin dakatarwa kuma an ƙare amsawa.Kasancewa ko rashi na Anti-SARS-CoV-2 neutralizing antibody a cikin samfurin an yanke hukunci ta hanyar ganowa (A-darajar) ta mai karanta microplate.