Anti-Ovarian (AO) Antibody ELISA Kit

Takaitaccen Bayani:

Ovary ya ƙunshi qwai, zona pellucida, granulosa cell, da dai sauransu, a matakai daban-daban na ci gaba. Kowane bangare na iya haifar da anti-ovarian antibodies (AoAb) saboda rashin bayyanar antigen. Zubewar antigen Ovarian wanda ya haifar da rauni na ovarian, kamuwa da cuta, ko kumburi na iya haifar da AoAb a cikin mutanen da ke da tabarbarewar rigakafi. AoAb yana ƙara lalata kwai kuma yana lalata aikin mahaifa da na mahaifa, yana haifar da rashin haihuwa da zubar da ciki.

An fara gano AoAb a cikin marasa lafiya tare da gazawar ovarian (POF) da farkon amenorrhea, mai alaƙa da halayen autoimmune. AoAb da farko yana rage haihuwa kuma a ƙarshe yana haifar da gazawar ovarian. Marasa lafiya marasa haihuwa tare da tabbataccen AoAb amma babu POF da zai iya fuskantar haɗarin POF mafi girma a nan gaba, yana buƙatar kimanta ajiyar ovarian.

AoAb positivity yana da girma a cikin marasa haihuwa da marasa haihuwa, yana nuna dangantaka ta kud da kud. Nazarin ya nuna AoAb yana haifar da rashin haihuwa fiye da zubar da ciki. Binciken na baya-bayan nan ya gano AoAb a yawancin marasa lafiya na PCOS, yana ba da shawarar ƙumburi na ovarian da ke haifar da rigakafi da cytokines mara kyau na iya haifar da PCOS da rashin haihuwa, wanda ke buƙatar ƙarin bincike.

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Cikakken Bayani

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Ka'ida

Wannan kit ɗin yana gano anti-ovarian antibodies (IgG) a cikin samfuran ruwan magani na ɗan adam dangane da hanyar kai tsaye, tare da ingantaccen antigens na ovarian membrane da aka yi amfani da shi don riga-kafi da microwells.

Tsarin gwaji yana farawa tare da ƙara samfurin magani zuwa rijiyoyin amsawar antigen-precoated don shiryawa. Idan anti-ovarian antibodies suna nan a cikin samfurin, za su ɗaure musamman ga antigens na ovarian membrane da aka rigaya a cikin microwells, suna samar da barga na antigen-antibody. Ana cire abubuwan da ba a ɗaure su ba don tabbatar da daidaiton ganowa.

Bayan haka, ana ƙara magungunan rigakafin cutar IgG mai suna horseradish peroxidase (HRP) a cikin rijiyoyin. Bayan shiryawa na biyu, waɗannan ƙwayoyin rigakafi masu lakabin enzyme suna ɗaure musamman ga ƙwayoyin rigakafin ovarian a cikin rukunin antigen-antibody ɗin da ke akwai, suna samar da cikakkiyar alamar rigakafin “antigen-antibody-enzyme”.

A ƙarshe, ana ƙara maganin substrate TMB. HRP a cikin hadaddun yana haifar da halayen sinadarai tare da TMB, yana haifar da canjin launi na bayyane. Ana auna abin sha (ƙimar A) na maganin amsawa ta hanyar amfani da mai karanta microplate, kuma kasancewar ko rashi na anti-ovarian antibodies a cikin samfurin an ƙaddara bisa ga sakamakon sha.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida	Enzyme da ke da alaƙa da immunosorbent assay
Nau'in	Kai tsayeHanya
Takaddun shaida	NMPA
Misali	Magungunan jini / plasma
Ƙayyadaddun bayanai	48T /96T
Yanayin ajiya	2-8℃
Rayuwar rayuwa	12watanni