Anti-Islet Cell (ICA) Antibody ELISA Kit

Takaitaccen Bayani:

An tsara wannan kit ɗin don gano in vitro matakan matakan anti-Islet cell antibody (ICA) a cikin maganin ɗan adam. A asibiti, ana amfani da shi azaman kayan aikin bincike na taimako don Nau'in Ciwon sukari na 1 (T1DM).

Kwayoyin rigakafin ƙwayoyin cuta na Islet sune autoantibodies waɗanda ke kai hari ga antigens akan saman ko cikin ƙwayoyin tsibiri na pancreatic, musamman ƙwayoyin β. Kasancewarsu yana da alaƙa da alaƙa da lalacewar autoimmune ga ƙwayoyin tsibiri, wanda shine mahimmin fasalin cututtukan ƙwayar cuta na T1DM. A farkon matakan T1DM, ko da kafin bayyanar cututtuka na asibiti kamar hyperglycemia, ana iya gano ICA sau da yawa a cikin jini, yana mai da shi muhimmiyar alamar rigakafi da wuri don cutar.

Ga mutanen da ke da tarihin iyali na ciwon sukari ko waɗanda ke nuna alamun pre-ciwon sukari, gano matakan ICA yana taimakawa tantance haɗarin haɓaka T1DM. Bugu da ƙari, a cikin marasa lafiya waɗanda ba su san abubuwan da ke haifar da hyperglycemia ba, gwajin gwajin ICA na taimaka wa bambance T1DM da sauran nau'in ciwon sukari, ta haka ne ke jagorantar tsara tsare-tsaren jiyya masu dacewa. Ta hanyar sa ido kan canje-canje a matakan ICA, kuma yana iya ba da tunani don kimanta ci gaban lalacewar ƙwayoyin tsibiri da tasirin matakan sa baki.

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Cikakken Bayani

Tags samfurin

Ka'ida

Wannan kit ɗin yana gano ƙwayoyin rigakafin ƙwayoyin cuta na tsibiri (ICA) a cikin samfuran jinin ɗan adam dangane da hanyar kai tsaye, tare da ingantaccen antigens cell tsibiri da ake amfani da su azaman antigen mai rufi.

Aikin gwajin yana farawa ta hanyar ƙara samfurin magani zuwa rijiyoyin da aka riga aka rufa su da antigen, sannan kuma shiryawa. Idan ICA ta kasance a cikin samfurin, za ta ɗaure ta musamman zuwa ga antigens cell cell mai rufi a cikin rijiyoyin, samar da barga na antigen-antibody. Ana cire abubuwan da ba a ɗaure su ba ta hanyar wankewa don tabbatar da daidaiton halayen da ke gaba.

Bayan haka, ana ƙara abubuwan haɗin enzyme zuwa rijiyoyin. Bayan matakin shiryawa na biyu, waɗannan abubuwan haɗin enzymes suna ɗaure zuwa rukunin antigen-antibody. Lokacin da aka gabatar da maganin substrate na TMB, enzyme a cikin hadaddun yana haifar da amsa tare da TMB, yana haifar da canjin launi na bayyane. A ƙarshe, ana amfani da mai karanta microplate don auna abin sha (A darajar), wanda ke ba da izini don ƙayyade matakan ICA a cikin samfurin dangane da tsananin tasirin launi.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida	Enzyme da ke da alaƙa da immunosorbent assay
Nau'in	Kai tsayeHanya
Takaddun shaida	NMPA
Misali	Magungunan jini / plasma
Ƙayyadaddun bayanai	48T /96T
Yanayin ajiya	2-8℃
Rayuwar rayuwa	12watanni