Anti-Endometrial (EM) Antibody ELISA Kit

Takaitaccen Bayani:

Ana amfani da wannan kit ɗin don gano qualitative in vitro na anti-endometrial antibodies (EmAb) a cikin maganin ɗan adam.

EmAb shine autoantibody wanda ke niyya na endometrium, yana haifar da martani na rigakafi. Yana da alamar antibody don endometriosis kuma yana da alaƙa da zubar da ciki na mace da rashin haihuwa. Rahotanni sun nuna 37% -50% na marasa lafiya da rashin haihuwa, zubar da ciki ko endometriosis sune EmAb-tabbatacce; adadin ya kai kashi 24% -61% a cikin mata bayan zubar da ciki ta wucin gadi.

EmAb yana ɗaure zuwa antigens na endometrial, yana lalata endometrium ta hanyar kunnawa da ɗaukar hoto na rigakafi, yana lalata dasa amfrayo da haifar da zubar ciki. Yana sau da yawa tare da endometriosis, tare da gano adadin 70% -80% a cikin irin waɗannan marasa lafiya. Wannan kit ɗin yana taimakawa bincikar endometriosis, lura da tasirin jiyya, da haɓaka sakamako don rashin haihuwa.

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Cikakken Bayani

Tags samfurin

Ka'ida

Wannan kit ɗin yana gano anti-endometrial antibodies (IgG) a cikin samfuran jini na ɗan adam dangane da hanyar kai tsaye, tare da ingantaccen antigens na membran endometrial da aka yi amfani da shi don riga-kafi da microwells.

Aikin gwajin yana farawa ta hanyar ƙara samfurin jini zuwa rijiyoyin amsawar antigen-precoated don shiryawa. Idan magungunan anti-endometrial sun kasance a cikin samfurin, za su ɗaure musamman ga antigens na endometrial da aka rigaya a cikin microwells, suna samar da barga na antigen-antibody. Bayan cire abubuwan da ba a ɗaure su ba ta hanyar wankewa don guje wa tsangwama, ana ƙara ƙwayoyin rigakafi na IgG mai suna horseradish peroxidase.

Bayan wani kumbura, waɗannan ƙwayoyin rigakafi masu lakabin enzyme suna ɗaure ga rukunin antigen-antibody. Lokacin da aka ƙara TMB substrate, wani launi yana faruwa a ƙarƙashin catalysis na enzyme. A ƙarshe, mai karanta microplate yana auna abin sha (A darajar), wanda ake amfani dashi don sanin kasancewar anti-endometrial antibodies (IgG) a cikin samfurin.

Siffofin Samfur

Babban hankali, ƙayyadaddun ƙayyadaddun ƙarfi da kwanciyar hankali

Ƙayyadaddun samfur

Ka'ida	Enzyme da ke da alaƙa da immunosorbent assay
Nau'in	Kai tsayeHanya
Takaddun shaida	NMPA
Misali	Magungunan jini / plasma
Ƙayyadaddun bayanai	48T /96T
Yanayin ajiya	2-8℃
Rayuwar rayuwa	12watanni