Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Wannan kit ɗin yana gano anti-cyclic citrullinated peptide antibodies (CCP antibodies) a cikin samfuran jini na ɗan adam dangane da hanyar kai tsaye, tare da tsaftataccen cyclic citrullinated peptide antigens da aka yi amfani da su azaman antigen mai rufi.

Tsarin gwajin yana farawa tare da ƙara samfurin magani zuwa rijiyoyin amsawa waɗanda aka riga aka yi musu rufi da tsaftataccen antigen da aka ambata, sannan lokacin shiryawa. A lokacin wannan shiryawa, idan ƙwayoyin rigakafi na CCP sun kasance a cikin samfurin, za su gane musamman kuma za su ɗaure ga peptide antigens na cyclic citrullinated wanda aka lulluɓe akan microwells, samar da barga na antigen-antibody complexes. Don tabbatar da daidaiton matakan da suka biyo baya, an cire abubuwan da ba a ɗaure ba a cikin rijiyoyin amsawa ta hanyar yin wanka, wanda ke taimakawa kawar da yuwuwar tsangwama daga wasu abubuwa a cikin maganin.

Bayan haka, ana ƙara conjugates enzyme zuwa rijiyoyin amsawa. Bayan shiryawa na biyu, waɗannan abubuwan haɗin enzymes za su haɗa musamman ga rukunin antigen-antibody da ke akwai, suna samar da babban hadaddun rigakafi wanda ya haɗa da antigen, antibody, da haɗin enzyme. Lokacin da TMB substrate bayani aka gabatar a cikin tsarin, da enzyme a cikin conjugate catalyzes wani sinadaran dauki tare da TMB substrate, haifar da bayyane launi canji. Ƙarfin wannan nau'in launi yana da alaƙa kai tsaye da adadin ƙwayoyin rigakafin CCP da ke cikin ainihin samfurin maganin. A ƙarshe, ana amfani da mai karanta microplate don auna abin sha (ƙimar A) na cakuda dauki. Ta hanyar nazarin wannan ƙimar sha, za a iya ƙayyade matakin ƙwayoyin rigakafi na CCP a cikin samfurin daidai, samar da ingantaccen tushe don gwajin asibiti da ya dace.