Ganewar HFRS - Zazzaɓin Hemorrhagic tare da Ciwon Renal

Fage

Kwayar cutar Hantaan (HV) ita ce cuta ta farko da ke da alhakin Zazzabin Hemorrhagic tare da Ciwon Renal (HFRS). HFRS cuta ce mai saurin yaduwa ta zoonotic a duniya wacce ke da zazzabi, zubar jini, da nakasar koda. Cutar tana da saurin farawa, saurin ci gaba, da kuma yawan mace-mace, wanda ke haifar da babbar barazana ga lafiyar jama'a. Rodents (irin su Apodemus agrarius da Rattus norvegicus) su ne manyan tafkunan ruwa da vectors na HV. Watsawa ga mutane yana faruwa da farko ta hanyar fitar da iska mai iska (fitsari, feces, miya), tuntuɓar kai tsaye, ko cizon vector. HFRS na iya faruwa a ko'ina cikin shekara, kuma yawan jama'a yana da saukin kamuwa. Bisa kididdigar da WHO ta yi, kasashe 32 a duniya sun ba da rahoton bullar cutar ta HV, musamman a gabashin Asiya, Turai, da yankin Balkan.

Alamar Antibody Bayan Kamuwar HV

Bayan kamuwa da cutar HV, tsarin rigakafi na ɗan adam yana samar da takamaiman ƙwayoyin rigakafi, da farko HV-IgM da HV-IgG.

HV-IgM rigakafi: Yi aiki azaman alamar serological na kamuwa da cuta da wuri, yawanci yana bayyana a cikin kwanaki na bayyanar cututtuka, kuma suna da mahimmanci don ganewar lokaci mai tsanani.

● Kwayoyin rigakafin HV-IgG: Fitowa daga baya kuma zai iya dawwama har tsawon rayuwa, yana nuna kamuwa da cuta da ta gabata ko kuma jin daɗi. Ƙaruwa mai ninki huɗu ko mafi girma a cikin HV-IgG antibody titer tsakanin m da convalescent samfurorin ruwan magani shima ana gano cutar kamuwa da cuta.

Hanyoyin Ganewar HV gama gari

Hanyoyin dakin gwaje-gwaje na yanzu don gano HV sun haɗa da keɓewar ƙwayoyin cuta, PCR, ELISA serological, da gwajin rigakafi na gwal na colloidal.

● Al'adun ƙwayoyin cuta da PCR suna ba da ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai amma suna ɗaukar lokaci, buƙata ta fasaha, kuma suna buƙatar ci-gaba na ɗakunan gwaje-gwaje, suna iyakance yawan amfani da su.

● Micro-immunofluorescence (MIF) yana ba da daidaito mai kyau amma yana buƙatar microscope mai haske da fassarar ƙwararru, ƙuntata aikace-aikacen yau da kullun.

● ELISA da colloidal zinariya assays an yarda da su sosai a cikin saitunan asibiti saboda sauƙi, saurin gudu, babban hankali da ƙayyadaddun ƙayyadaddun, da sauƙi na tarin samfurin (serum / plasma).

Ayyukan Samfur

Beier Bio's HV-IgM/IgG (ELISA) Fasalolin Assay

● Nau'in Samfura: Serum, plasma

● Samfurin Dilution: Dukansu IgM da IgG suna amfani da samfurin asali mai kyau tare da 1: 11 dilution (100µl samfurin diluent + 10µl samfurin), daidaita aikin aiki da rage yawan aikin ma'aikaci.

● Reagent Shirye-Don Amfani: Duk reagents suna shirye ban da buffer mai wankewa (20× mai da hankali). Launi mai launi don ganewa cikin sauƙi

● Tsarin Ƙaddamarwa: 30 min / 30 min / 15 min; cikakken atomatik

● Tsawon Tsayin Gano: 450 nm tare da nuni na 630 nm

● Rijiyoyi masu rufi: rijiyoyi 96 ko 48 masu karyewa, kowannensu yana da lambar samfur da aka buga don ganowa da dacewa.

Siffofin Assay na Beier Bio's HV-IgM/IgG (Colloidal Gold)

● Nau'in Misali: Magani

● Lokacin Ganewa: Sakamako a cikin mintuna 15; babu ƙarin kayan aiki da ake buƙata; manufa don saurin dubawa a cikin marasa lafiya, gaggawa, da saitunan marasa lafiya tarwatsa

● Tsari: Ƙara samfurin 10µl zuwa samfurin katin gwaji da kyau ta amfani da dropper; fassara sakamakon a cikin mintuna 15-20

Ayyukan Clinical na HV-IgM (ELISA), HV-IgG (ELISA), da HV-IgM/IgG (Colloidal Gold)